Extraction of the wheat straw hemicellulose fraction assisted by commercial endo-xylanases. Role of the accessory enzyme activities

Wheat straw is a highly promising raw material for bio-refinery strategies. Most of the literature related to lignocellulose fractionation focuses on cellulose purification and hemicellulose solubilization. Pre-treatments solubilization without formation undesired products usually reach low extraction yields, which leaves an important fraction unused. In this work, we propose mild process efficient wheat assisted by partial enzymatic hydrolysis with three commercial endo-xylanase cocktails. A first step alkali at 40 ºC helped disrupt lignocellulosic structure removed 19% lignin while maintaining most in solid. The enabled reaching yields 59.8%, 51.9%, 42.5% Ultraflo L, Pentopan mono conc, Shearzyme 500L, respectively. We also discuss catalytic properties each endo-xylanase, particular, their adscription GH10 or GH11 glycosyl hydrolase family, relevant role accessory enzymes.